thelifeofapremed:

Post-Transcriptional Processing

Only in the Nucleus for Eukaryotes
Primary transcript processed in 3 ways:
1. ADDition of nucleotides
2. DELETion of nucleotides
3. Modification of Nitrogenous Bases
5’ cap and 3’ Poly A tail
snRNP and the Spliceosome of Doom!
INtrons = IN nucleus
EXtrons = EXit the nucleus

DNA TECHNOLOGY

Clone Library = plasmid and replication

Polymerase chain reaction (PCR) : ‘fast’ cloning

Heat to denature
Mix with primers, let cool = primers hybridize
Add polymerase to amplify complementary strands

Southern Blot

1. Chop up DNA
2. Efield to spread out pieces by SIZE
3. Blot it!
4. Add Radioactive DNA or RNA Probe
5. Visualize on radiographic film 
GENETIC CODE
Degenerative – more than one series of nucleotides may code for ANY A.A.
Unambigous = one series of nucleotides = one A.A.
Universal code!
START! AUG
STOP! UAA, UAG, UGA
4^3 = 64 
Ex. protein of 100 A.A. = there are 20^100 possible amino acids sequences for the protein 
  • People who can't draw: Drawing is fucking hard
  • People who don't try at all: Drawing is fucking hard
  • Teachers: Drawing is fucking hard
  • Beginning artists: Drawing is fucking hard
  • Pro artists: Drawing is fucking hard
  • Famous artists: Drawing is fucking hard
  • Extremely famous artists: Drawing is fucking hard
  • Long gone, passed away artists who went down in history: Drawing is fucking hard
  • People who are upset an artist won't draw for them for free: Drawing is easy!

Tags: bless

rfmmsd:

Artist:
Martin Wittfooth
"Rajas (The Age of Energy)"
Oil on Panel
24” x 20”
2014

rfmmsd:

Artist:

Martin Wittfooth

"Rajas (The Age of Energy)"

Oil on Panel

24” x 20”

2014

(Source: martinwittfooth.com)

wayfaringmd:

In a hospital follow up visit with T, one of my favorite patients, whose stroke left her completely without a conversation filter.

T: Dr. Wayfaring! What are all these dark spots on me? I got all bruised up in the hospital and they turned dark and they won’t go away.

Me: Well sometimes people get what’s called post-inflammatory hyperpigmentation, where the skin gets darker after it’s bruised or broken. It can take a long time to fade. It’s more common in people with darker skin tones.

T: Wayfaring, you callin me black!?

image

Me: Um… well… I’m saying that people of many races with darker skin get this more commonly.

T: So you callin me black then. 

image

Me: No, but…well… you are black. 

T: image

AHAHAHAHAHAHA! Girl I’m just playing with you!

Me: image

Geez! I thought we were friends, T! Don’t go messin with me like that!

T: But Dr. Wayfaring, for real tho, what are you

Me: Whaddya mean what am I?

T: I mean I’m black but what are you? 

Me: Uh, white…

T: nah, you ain’t white. 

Me: ok, then what am I?

image

T: You paaaaaale. Girl you need a tan like REAL bad.

Me: True, true. I am very white.

image

T: Nah, you ain’t white, cuz white people be trippin. But I like you though.

soprie:

Stop calling what’s happening in Ferguson a “riot”.

It is not a riot.

Vancouver losing the Stanley Cup a few years ago was a riot. It was angry, drunken destruction with no purpose. (And as a Canadian, it was a shameful event)

Ferguson is not a riot. It is a protest. It is an uprising. It is a civil rights revolution. The prople of Ferguson may be angry, but they have a reason to be angry, and they are not violent, and they are not hooligans, thugs or looters. They are protesting for their human rights which are currently being denied.

Look at the difference between a riot and a protest. A riot is chaos. A protest has a purpose.

1 2

(via tyleroakley)

kaddy-kablamo:

"They didn’t allow me to become a nurse because of my hijab. Thank you, I became a doctor instead" #BURN

kaddy-kablamo:

"They didn’t allow me to become a nurse because of my hijab. Thank you, I became a doctor instead" #BURN

(via thisfuturemd)

prepaidafrica:

Catherine Kaluwa, a PhD student from the University of Nairobi, Kenya, talks about the journey she has taken from walking barefoot to school to researching the efficacy of plants that women in her homeland have traditionally taken for family planning. She also outlines some of the challenges she has faced as a female researcher working in a male-dominated profession.

Via Timbuktu Chronicles

(via theafricatheynevershowyou)

bellecosby:

whoa-bruh:

bellecosby:

My goal in life is to make a nigga feel the way Terrance Howard was feeling when Beyonce was giving him a lap dance 

image

SAME.

NEW GOAL

image

image

I’M TRYNA HAVE A NIGGA QUESTIONING HIS WHOLE LIFE EXISTENCE LIKE NICKI HAD DRAKE AFTER SHE GAVE HIM A LAPDANCE 

(via johnnysmilesduh)

thebrainscoop:

Science Needs Women: 
For Women in Science; the L’Oreal Foundation 

I’m sharing this video on any platform I can because when I first found it last week it had something like 1,400 views, but it’s the most beautifully produced and succinctly narrated video addressing some of the most complicated issues facing women in STE(A)M fields I’ve found yet. 

I’m sharing this for every time I’m called a “feminazi.”

…for every time I’m told that my concerns aren’t valid, our that our issues are imagined.

…for every time I hear “women just don’t like science,” or worse - “women just aren’t good at science.”

…for every time we’re told that we can have a family or a career, but not both - and for every time we feel like we have to decide between the two.

…for every time a study comes out saying as many as 64% of women endure sexual harassment during field work

…for the fact that women earn 41% of PhD’s in STEM fields, but make up only 28% of tenure-track faculty in those fields.

…and because we need more women mentors in these fields to stand up for issues that are not “women’s issues” - these are people issues that affect our collective society as a whole.

The women in this video are my heroes and they should be your heroes, too.

"

Why is it that people are willing to spend $20 on a bowl of pasta with sauce that they might actually be able to replicate pretty faithfully at home, yet they balk at the notion of a white-table cloth Thai restaurant, or a tacos that cost more than $3 each? Even in a city as “cosmopolitan” as New York, restaurant openings like Tamarind Tribeca (Indian) and Lotus of Siam (Thai) always seem to elicit this knee-jerk reaction from some diners who have decided that certain countries produce food that belongs in the “cheap eats” category—and it’s not allowed out. (Side note: How often do magazine lists of “cheap eats” double as rundowns of outer-borough ethnic foods?)

Yelp, Chowhound, and other restaurant sites are littered with comments like, “$5 for dumplings?? I’ll go to Flushing, thanks!” or “When I was backpacking in India this dish cost like five cents, only an idiot would pay that much!” Yet you never see complaints about the prices at Western restaurants framed in these terms, because it’s ingrained in people’s heads that these foods are somehow “worth” more. If we’re talking foie gras or chateaubriand, fair enough. But be real: You know damn well that rigatoni sorrentino is no more expensive to produce than a plate of duck laab, so to decry a pricey version as a ripoff is disingenuous. This question of perceived value is becoming increasingly troublesome as more non-native (read: white) chefs take on “ethnic” cuisines, and suddenly it’s okay to charge $14 for shu mai because hey, the chef is ELEVATING the cuisine.

"

— One of the entries from the list ‘20 Things Everyone Thinks About the Food World (But Nobody Will Say)’. (via crankyskirt)

(via bootydeficit)

Tags: bless

scienceyoucanlove:

Pulmonary alveoli seen under the microscope An alveolus (plural: alveoli, from Latin alveolus, “little cavity”) is an anatomical structure that has the form of a hollow cavity. Found in the lung parenchyma, the pulmonary alveoli are the terminal ends of the respiratory tree, which outcrop from either alveolar sacs or alveolar ducts, which are both sites of gas exchange with the blood as well.Image by David Gregory and Debbie MarshallFound on anatomybox.com
text source: Daily Anatomy

scienceyoucanlove:

Pulmonary alveoli seen under the microscope 

An alveolus (plural: alveoli, from Latin alveolus, “little cavity”) is an anatomical structure that has the form of a hollow cavity. Found in the lung parenchyma, the pulmonary alveoli are the terminal ends of the respiratory tree, which outcrop from either alveolar sacs or alveolar ducts, which are both sites of gas exchange with the blood as well.

Image by David Gregory and Debbie Marshall
Found on anatomybox.com

text source: Daily Anatomy

shanitasims:

Mamou - preview for upcoming editorial on CURB APPEAL. Soon…

shanitasims:

Mamou - preview for upcoming editorial on CURB APPEAL. Soon…

(via shadesofblackness)

Tags: style

ewebean:

livelong-and-dftba:

capleesi:

SHOTS FIRED

Well we all remember what happened the last time someone challenged him.

image

daniel no baby you don’t know what you just did he is going to eAT YOU

(via thesnowqueer)

Tags: hp lotr